Webb1 juni 2013 · HIPK2 is a member of the evolutionarily conserved family of homeodomain-interacting protein kinases (HIPKs) that includes three highly homologous S/T kinases originally identified for their capacity to interact with homeodomain transcription factors ( i.e., HIPK1, HIPK2, and HIPK3) [27], and a fourth member (HIPK4), that has been … WebbHere we describe a mechanism through which HMGA1 inhibits p53-mediated apoptosis by counteracting the p53 proapoptotic activator homeodomain-interacting protein kinase 2 (HIPK2). We found that HMGA1 overexpression promoted HIPK2 relocalization in the cytoplasm and inhibition of p53 apoptotic function, while HIPK2… Expand
High Mobility Group A1 (HMGA1) proteins regulate p53-mediated …
Webb1 mars 2007 · HIPK2 nuclear localization strongly increases HIPK2-induced apoptosis. ( A) Western blot analyses of endogenous HMGA1, HIPK2, and p53 in WT p53–carrying RKO cells transduced with HMGA1-specific sense or antisense oligonucleotides. γ-Tubulin was used as loading control. WebbHIPK2 relocalization from the nucleus to the cytoplasm, in- In contrast, in neoplastically transformed cells as well as in hibiting p53 apoptotic function, whereas HIPK2 overexpres- embryonic cells, the constitutive expression of HMGA pro- sion re-established HIPK2 nuclear localization and teins is exceptionally high (5). phone hake app
HIPK family kinases bind and regulate the function of the CCR4 …
WebbFigure 3 Contribution of p53 phosphorylation at Ser46 to HMGA1/HIPK2-induced BAX promoter activity. (A) Western blot analysis of the indicated protein in WT p53– carrying HCT116 cells 48 hours after infection at the indicted MOI. (B) The same cells analyzed in A were treated with UV light at 50 J/m2. At 5 and 12 hours after UV light irradiation, the … WebbHIPK2 binds and phosphorylates histone H2B at S14 (H2B-S14P), and the two proteins colocalize at the midbody. ... High-mobility group A1 inhibits p53 by cytoplasmic relocalization of its proapoptotic activator HIPK2. J. Clin. Invest. 2007; 117: 693-702. Crossref; PubMed; Scopus (82) Google Scholar, Wee et al., 2008. Wee H.J. Voon D.C. Webboverexpression, HIPK2 cytoplasmic localization, and low spontaneous apoptosis index (comparable to that observed in mutant p53–carrying tumors) was observed in WT p53–expressing human breast carcinomas. Hence, cytoplasmic relocalization of HIPK2 induced by HMGA1 overexpression is a mechanism of inactiva- phone hacking tools for pc